Kinesin molecular motor Eg5 functions during polypeptide synthesis
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چکیده
منابع مشابه
Kinesin molecular motor Eg5 functions during polypeptide synthesis
The kinesin-related molecular motor Eg5 plays roles in cell division, promoting spindle assembly. We show that during interphase Eg5 is associated with ribosomes and is required for optimal nascent polypeptide synthesis. When Eg5 was inhibited, ribosomes no longer bound to microtubules in vitro, ribosome transit rates slowed, and polysomes accumulated in intact cells, suggesting defects in elon...
متن کاملcoTitle: Kinesin Molecular Motor Eg5 Functions During the Elongation Phase of Polypeptide Synthesis
The kinesin-‐related molecular motor Eg5 plays a well-‐defined role in cell division, promoting spindle assembly, but here we show that during interphase Eg5 primarily associated with ribosomes and both Eg5 and the microtubule cytoskeleton were required for optimal nascent polypeptide ...
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Cell division is an essential process which was prerequisite for the development of life and is key for both development of complex organisms and reproduction in general. e accuracy of this process, which is a part of mitosis, depends on the mitotic spindle, a bipolar, dynamic structure which segregates sister chromatids and thus separates the genomes of the two developing daughter cells. Its s...
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Monastrol is a small, cell-permeable molecule that arrests cells in mitosis by specifically inhibiting Eg5, a member of the Kinesin-5 family. We have used steady-state and presteady-state kinetics as well as equilibrium binding approaches to define the mechanistic basis of S-monastrol inhibition of monomeric human Eg5/KSP. In the absence of microtubules (Mts), the basal ATPase activity is inhib...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 2011
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.e11-03-0211